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strain, we recognized genes involved with early stages of biofilm formation. One course of mutations recognized in this review mapped within a cluster of genes specifying the elements of the chaperone/usher pathway that is certainly involved in assembly of fimbrial subunits in other microorganisms. These genes, not Beforehand explained in P. aeruginosa
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We also examined the Corporation of genes that specify adhesins of your chaperone/usher household in other bacteria. We noticed that a gene cluster in Y. pestis
gene was obtained through microscopic Evaluation of microorganisms connected to glass protect slides (see Materials and Strategies
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The DNA sequences acquired were being analyzed Together with the blastn plan and when compared Using the PAO1 genome at . The putative ORFs ended up then analyzed with ORF finder (). The annotated genome was used on release to update our Investigation.
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. Considering that the Examination of the relatedness on the CupA proteins to other fimbrial systems with outlined constructions, like the thick or slim filaments (14), disclosed no consistent pattern, we should always look depo 50 bonus 50 at the possibility that the CupA proteins participate in the assembly of a style of adhesive organelle not previously recognized.